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Western blot analysis of NOX3 in 293 cell lysate with NOX1 antibody at 1 μg/ml.
Immunohistochemistry of NOX3 in human kidney tissue with NOX3 antibody at 5 μg/ml.
Immunofluorescence of NOX3 in human kidney tissue with NOX3 antibody at 20 μg/ml.
Western blot analysis of NOX3 in 293 cell lysate with NOX1 antibody at 1 μg/ml.
Trial Size

NOX3 Antibody

CATALOG NUMBER: 7925

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
NOX3 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX3.
The immunogen is located within amino acids 110 - 160 of NOX3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NOX3 antibody can be used for detection of NOX3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
NOX3 antibody is human and mouse reactive. NOX3 is predicted to not cross-react with other NOX proteins.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Predicted Molecular Weight:
Predicted: 62 kDa
Observed: 72 kDa
Purification:
NOX3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NOX3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NOX3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
NOX3
Additional Names:
NAPDH oxidase 3, NOX-3, GP91-3, MOX2, MOX-2
Protein Accession Number:
NP_056533
Protein Gi Number:
11136626
Ncbi Gene Id Number:
50508
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX3 is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia/otolith (2,3). It has been suggested that NOX3 is activated by the Transient Receptor Potential Vanilloid 1 (TRPV1), and this activity causes increased levels of reactive oxygen species in the inner ear, which in turn leads to STAT1-mediated inflammation and hearing loss (4).
Background Reference 1:
Bedard K and Krause KH. The Nox family of ROS-generating NAPDH oxidases: physiology and pathophysiology. Physiol. Rev. 2007; 87:245-313.
Background Reference 2:
Cheng G, Cao Z, Xu X, et al. Homologs of gp91phox: cloning and tissue expression of Nox3, Nox4, and Nox5. Gene 2001; 269:131-40.
Background Reference 3:
Paffenholz R, Bergstrom RA, Pasutto F, et al. Vestibular defects in head-tilt mice result from mutations in Nox3, encoding an NADPH oxidase. Genes Dev. 2004; 18:486-91.
Background Reference 4:
Mukherjea, Jajoo S, Sheehan K, et al. NOX3 NADPH oxidase couples transient receptor potential vanilloid 1 to signal transducer and activator of transcription 1-mediated inflammation and hearing loss. Antioxidants and Redox Signaling 2011; 14:999-1010.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7925

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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