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Western blot analysis of NOX4 in Jurkat cell lysate with NOX4 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of NOX4 in human spleen tissue with NOX4 antibody at 5 μg/ml.
Immunofluorescence of NOX4 in human spleen tissue with NOX4 antibody at 20 μg/ml.
Western blot analysis of NOX4 in Jurkat cell lysate with NOX4 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

NOX4 Antibody

CATALOG NUMBER: 7927

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
NOX4 antibody was raised against a 14 amino acid peptide near the amino terminus of human NOX4.
The immunogen is located within amino acids 70 - 120 of NOX4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NOX4 antibody can be used for detection of NOX4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
NOX4 antibody is human, mouse, and rat reactive. At least four isoforms of NOX4 are known to exist. NOX4 is predicted to not cross-react with other NOX proteins.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 64 kDa
Observed: 68 kDa
Purification:
NOX4 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NOX4 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NOX4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
NOX4
Additional Names:
NAPDH oxidase 4, NOX-4, RENOX, KOX, KOX-1
Protein Accession Number:
NP_058627
Protein Gi Number:
8393843
Ncbi Gene Id Number:
50507
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX4 is is expressed in multiple tissues and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS) (2,3). Unlike other NOX proteins, NOX4 does not require cytosolic subunits and thus is constitutively active (4). The function of NOX4 remains unclear as it plays both protective and deleterious roles in cellular metabolism.
Background Reference 1:
Bedard K and Krause KH. The Nox family of ROS-generating NAPDH oxidases: physiology and pathophysiology. Physiol. Rev. 2007; 87:245-313.
Background Reference 2:
Cheng G, Cao Z, Xu X, et al. Homologs of gp91phox: cloning and tissue expression of Nox3, Nox4, and Nox5. Gene 2001; 269:131-40.
Background Reference 3:
Montezano AC, Burger D, Ceravolo GS, et al. Novel Nox homologues in the vasculature: focusing on Nox4 and Nox5. Clin. Sci. 2011; 120:131-41.
Background Reference 4:
Martyn KD, Frederick LM, von Loehneysen K, et al. Functional analysis of Nox4 reveals unique characteristics compared to other NAPDH oxidases. Cell. Signal. 2006; 18:69-82.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7927

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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