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45-979 (10ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-979 (10ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

NOXO1 Antibody

CATALOG NUMBER: 45-979

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: RGCVDSVPHPTTEQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave no signal but low background in Hela and Human Heart lysates at up to 1ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney Cortex shows cytoplasmic stain in epithelial cells of some PCT. Recommended concentration, 10-20ug/ml.
Specificity:
This antibody is expected to recognise all three reported isoforms (as represented by NP_653204.1, NP_751907.1and NP_751908.1).
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NOXO1
Additional Names:
NOXO1, P41NOX, P41NOXA, P41NOXB, P41NOXC, MGC20258, NADPH oxidase organizer 1, regulatory protein P41NOX, SH3PXD5, SNX28, Nox organizer 1
Protein Accession Number:
NP_653204.1,NP_751907.1,NP_751907.1
Protein Gi Number:
21389405
Ncbi Gene Id Number:
124056
Background Reference 1:
Banfi B, Clark RA, Steger K, Krause KH. Two novel proteins activate superoxide generation by the NADPH oxidase NOX1. J Biol Chem. 2003 Feb 7;278(6):3510-3.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-979

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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