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Western blot analysis of NPAS3 in SK-N-SH cell lysate with NPAS3 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunohistochemistry of NPAS3 in human brain tissue with NPAS3 antibody at 5 μg/mL.
Immunofluorescence of NPAS3 in human brain tissue with NPAS3 antibody at 20 μg/mL.
Western blot analysis of NPAS3 in SK-N-SH cell lysate with NPAS3 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Trial Size

NPAS3 Antibody

CATALOG NUMBER: 4107

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (84%), Chicken: (80%)
Immunogen:
NPAS3 antibody was raised against a 28 amino acid synthetic peptide from near the amino terminus of human NPAS3.
The immunogen is located within amino acids 40 - 90 of NPAS3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NPAS3 antibody can be used for detection of NPAS3 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 2:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 3:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
NPAS3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NPAS3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NPAS3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NPAS3
Additional Names:
NPAS3 Antibody: MOP6, PASD6, bHLHe12, BHLHE12, MOP6, Neuronal PAS domain-containing protein 3, Basic-helix-loop-helix-PAS protein MOP6, Neuronal PAS3
Protein Accession Number:
Q8IXF0
Protein Gi Number:
38604888
Ncbi Gene Id Number:
64067
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NPAS3 Antibody: Neuronal PAS domain protein 3 (NPAS3) is a brain-enriched basic helix-loop-helix PAS domain transcription factor and is broadly expressed in the developing neuroepithelium and has recently found to be disrupted by genetic translocation in a family affected with schizophrenia. It was recently shown to be involved in the regulation of FGF signaling in the dentate gyrus by controlling the expression of the FGF receptor subtype 1 and in turn neurogenesis emanating from this region. NPAS3-null mice were growth-retarded and displayed brain defects that included reduced size of the anterior hippocampus, hypoplasia of the corpus callosum, and enlargement of the ventricles, as well as several behavioral abnormalities. Furthermore, these NPAS3-null mice also exhibited disruptions in several neurosignaling pathways involving glutamate, dopamine, and serotonin. These results demonstrate the essential role played by NPAS3 during structural and functional brain development. At least three isoforms of NPAS3 are known to exist.
Background Reference 1:
Brunskill EW, Witte DP, Shreiner AB et al. Characterization of npas3, a novel basic helix-loop-helix PAS gene expressed in the developing mouse nervous system. Mech. Dev.1999; 88:237-41.
Background Reference 2:
Kamnasaran D, Muir WJ, Ferguson-Smith MA, et al. Disruption of the neuronal PAS3 gene in a family affected with schizophrenia. J. Med. Genet.40:325-32.
Background Reference 3:
Brunskill EW, Ehrman LA, Williams MT, et al. Abnormal neurodevelopment, neurosignaling and behaviour in Npas3-deficient mice. Euro. J. Neurosci.2005; 22:1265-76.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4107

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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