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Western blot analysis of extracts of mouse lung, using NPC2 antibody (19-648) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using NPC2 Antibody (19-648) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using NPC2 Antibody (19-648) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using NPC2 Antibody (19-648) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of mouse lung, using NPC2 antibody (19-648) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

NPC2 Antibody

CATALOG NUMBER: 19-648

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-151 of human NPC2 (NP_006423.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
Mouse lung
Predicted Molecular Weight:
Observed: 19kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NPC2
Additional Names:
NPC2, tissue-specific secretory protein, NP-C2, Niemann-Pick disease, type C2, MGC1333, HE1, epididymal secretory protein E1, epididymal secretory protein, epididymal protein 1, EDDM1
Ncbi Gene Id Number:
10577
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-648

List Size:
100 uL

List Price:

$436.00

Datasheet
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