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Western blot analysis in K562 cell line lysates (35ug/lane).
Western blot analysis in K562 cell line lysates (35ug/lane).

NPFFR1 Antibody

CATALOG NUMBER: 57-553

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NPFFR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 337-366 amino acids from the C-terminal region of human NPFFR1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
48 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NPFFR1
Additional Names:
Neuropeptide FF receptor 1, G-protein coupled receptor 147, RFamide-related peptide receptor OT7T022, NPFFR1, GPR147, NPFF1
Protein Accession Number:
Q9GZQ6
Protein Gi Number:
13878599
Ncbi Gene Id Number:
64106
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Ubuka, T., et al. PLoS ONE(12), E8400 (2009) :
Background Reference 3:
Dowal, L., et al. J. Biol. Chem. 281(33):23999-24014(2006)
Background Reference 4:
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-553

List Size:
400 ul

List Price:

$495.00

Datasheet
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