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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
NPHP3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).

NPHP3 Antibody

CATALOG NUMBER: 56-625

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Xenopus
Immunogen:
This NPHP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-173 amino acids from the N-terminal region of human NPHP3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
151 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NPHP3
Additional Names:
Nephrocystin-3, NPHP3, KIAA2000
Protein Accession Number:
Q7Z494
Protein Gi Number:
68565783
Ncbi Gene Id Number:
27031
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein containing a coiled-coil (CC)domain, a tubulin-tyrosine ligase (TTL) domain, and a tetratricopeptide repeat (TPR) domain. The encoded protein interacts withnephrocystin and may function in renal tubular development andfunction. Mutations in this gene are associated withnephronophthisis type 3. Multiple splice variants have beendescribed but their full-length nature has not been determined.
Background Reference 1:
Simpson, M.A., et al. Am. J. Kidney Dis. 53(5):790-795(2009)
Background Reference 2:
Tory, K., et al. Kidney Int. 75(8):839-847(2009)
Background Reference 3:
Bergmann, C., et al. Am. J. Hum. Genet. 82(4):959-970(2008)
Background Reference 4:
Hoefele, J., et al. J. Am. Soc. Nephrol. 18(10):2789-2795(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-625

List Size:
400 ul

List Price:

$495.00

Datasheet
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