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Western blot analysis of SMMC - 7721 cell extracts with NPM1 antibody.
Immunohistochemical staining of paraffin embedded human liver carcinoma showing nuclear localization NPM1 atnibody with DAB staining.
Western blot analysis of SMMC - 7721 cell extracts with NPM1 antibody.

NPM1 Antibody [7H10B9]

CATALOG NUMBER: 32-198

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein A affinity chromatography.
Clonality:
Monoclonal
Isotype:
IgG1
Physical State:
Liquid
Concentration:
batch dependent
Storage Conditions:
NPM1 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NPM1
Additional Names:
NPM, Nucleolar phosphoprotein B23, NPM, B23
Protein Accession Number:
P06748
Protein Gi Number:
114762
Ncbi Gene Id Number:
4869
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Nucleophosmin (NPM), also named B23 or NO38, is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. NPM1 has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication and molecular chaperoning activities . The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase .
Background Reference 1:
Morris SW, et al. Science 1994 Mar 4; 263(5151):1281-4.
Background Reference 2:
Yoneda-Kato N, et al. Oncogene. 1996 Jan 18;12(2):265-75.
Background Reference 3:
Liu HT, Yung BY. Cancer Lett. 1999 Sep 20;144(1):45-54.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-198

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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