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42-909 (1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-909 (2ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
42-909 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
42-909 (10ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-909 (1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NPR3 Antibody

CATALOG NUMBER: 42-909

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-HRELREDSIRSHFS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 52kDa band observed in Human Placenta lysates (calculated MW of 59.8kDa according to NP_000899.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows staining of brush borders in select PCT. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Prostate. Recommended concentration: 10ug/ml.
Specificity:
The immunizing peptide represents the cytoplasmic part of the protein.
Positive Control 1:
Cat. No. 1309 - Human Placenta Lysate
Predicted Molecular Weight:
Approx 52 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NPR3
Additional Names:
NPR3, natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C), ANPRC, GUCY2B, NPRC, ANP-C, ANPR-C, NPR-C, OTTHUMP00000197048, OTTHUMP00000220980, atrial natriuretic peptide clearance receptor, atrial natriuretic peptide receptor 3, atrial natriuretic peptide receptor type C
Protein Accession Number:
NP_000899.1
Protein Gi Number:
4505441
Ncbi Gene Id Number:
4883

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-909

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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