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NPT Antibody [4B4D1]

CATALOG NUMBER: 32-199

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:Dilution 1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein A affinity chromatography.
Clonality:
Monoclonal
Isotype:
IgG2a
Physical State:
Liquid
Concentration:
batch dependent
Storage Conditions:
NPT monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
npt
Protein Accession Number:
Q79C30
Protein Gi Number:
81919445
Ncbi Gene Id Number:
11979140
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.
Background Reference 1:
Landis WG et.al Human and ecological Risk Assessment 6(5): 875-899.
Background Reference 2:
Stewart CN et.al BioTechniques 29(4): 832-843.
Background Reference 3:
Thomson JA et.al Journal of Food Science 66(2): 188-193.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-199

List Size:
0.1 mg

List Price:

$408.00

Datasheet
Shipping Info

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