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45-981 (1ug/ml) staining of Rat (A) and Pig (B) Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence
45-981 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-981 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
45-981 staining (0.1ug/ml) of U251 cell lysate (RIPA buffer, 35ug total protein per lane). Detected using chemiluminescence.
45-981 staining (0.03ug/ml) of Human Kidney (A) and (0.3ug/ml) Lung (B) lysate (RIPA buffer, [35ug total protein per lane). Detected using chemiluminescence.
45-981 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
45-981 (1ug/ml) staining of Rat (A) and Pig (B) Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence

NQO1 Antibody

CATALOG NUMBER: 45-981

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-SIPTDNQIKARK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 28kDa band observed in lysates of Glioblastoma cell line U251 and approx 30kDa band observed in Human Kidney and Lung lysates and in Rat and Pig Kidney lysates (calculated MW of 27.3kDa according to Human NP_001020604.1 and 30.9kDa according to Human NP_000894.1, Rat NP_058696.2 and Pig NP_001153085.1). Recommended concentration: 0.03-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 and U251 cells. Recommended concentration: 5-10ug/ml.
Specificity:
This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1).
Positive Control 1:
Cat. No. 1302 - Human Lung Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NQO1
Additional Names:
NQO1, NAD(P)H dehydrogenase, quinone 1, DTD, QR1, DHQU, DIA4, NMOR1, NMORI, diaphorase-4, diaphorase (NADH/NADPH), diaphorase (NADH/NADPH) (cytochrome b-5 reductase), NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1, DT-diaphorase, NAD(P)H menadione oxidoreductase 1, dioxin-inducible, NAD(P)H:Quinone acceptor oxidoreductase type 1, NAD(P)H menadione oxidoreductase 1, dioxin-inducible, NAD(P)H:quinone oxireductase, azoreductase, diaphorase (NADH/NADPH) (cytochrome b-5 reductase), dioxin-inducible 1, menadione reductase, phylloquinone reductase, quinone reductase 1
Protein Accession Number:
NP_000894.1,NP_001020604.1,NP_001020605.1
Protein Gi Number:
4505415
Ncbi Gene Id Number:
1728
Background Reference 1:
Asher G, Lotem J, Kama R, Sachs L, Shaul Y. NQO1 stabilizes p53 through a distinct pathway. Proc Natl Acad Sci USA. 2002 Mar 5;99(5):3099-104.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-981

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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