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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

NR0B1 Antibody

CATALOG NUMBER: 30-344

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR0B1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NR0B1 antibody can be used for detection of NR0B1 by ELISA at 1:312500. NR0B1 antibody can be used for detection of NR0B1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
52 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NR0B1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NR0B1
Additional Names:
NR0B1, AHC, AHCH, AHX, DAX-1, DAX1, DSS, GTD, HHG, NROB1, SRXY2
Protein Accession Number:
NP_000466
Protein Gi Number:
5016090
Ncbi Gene Id Number:
190
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NR0B1 is a protein that contains a DNA-binding domain. The protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in its gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.
Background Reference 1:
Mantovani, G., (2005) Fertil. Steril. 84 (5), 1542-1544.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-344

List Size:
100 ul

List Price:

$519.00

Datasheet
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