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Western blot analysis in Hela cell line lysates (35ug/lane).
Western blot analysis of NR0B1 using rabbit polyclonal NR0B1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NR0B1 gene.
NR0B1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Hela cell line lysates (35ug/lane).

NR0B1 Antibody

CATALOG NUMBER: 57-262

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NR0B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-333 amino acids from the Central region of human NR0B1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
52 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NR0B1
Additional Names:
Nuclear receptor subfamily 0 group B member 1, DSS-AHC critical region on the X chromosome protein 1, Nuclear receptor DAX-1, NR0B1, AHC, DAX1
Protein Accession Number:
P51843
Protein Gi Number:
20532385
Ncbi Gene Id Number:
190
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that contains a DNA-bindingdomain. The encoded protein acts as a dominant-negative regulatorof transcription which is mediated by the retinoic acid receptor.This protein also functions as an anti-testis gene by actingantagonistically to Sry. Mutations in this gene result in bothX-linked congenital adrenal hypoplasia and hypogonadotropichypogonadism.
Background Reference 1:
Li, N., et al. J. Clin. Endocrinol. Metab. 95 (9), E104-E111 (2010) :
Background Reference 2:
Nedumaran, B., et al. J. Biol. Chem. 285(12):9221-9232(2010)
Background Reference 3:
Kinsey, M., et al. Cancer Res. 69(23):9047-9055(2009)
Background Reference 4:
Nagl, F., et al. Am. J. Physiol., Cell Physiol. 297 (5), C1146-C1156 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-262

List Size:
400 ul

List Price:

$495.00

Datasheet
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