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Western blot analysis in HepG2 cell line lysates (35ug/lane).This demondetected the NR0B2 protein (arrow).
NR0B2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of NR0B2 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in HepG2 cell line lysates (35ug/lane).This demondetected the NR0B2 protein (arrow).

NR0B2 Antibody

CATALOG NUMBER: 55-606

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NR0B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-83 amino acids from the Central region of human NR0B2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NR0B2
Additional Names:
Nuclear receptor subfamily 0 group B member 2, Orphan nuclear receptor SHP, Small heterodimer partner, NR0B2, SHP
Protein Accession Number:
Q15466
Protein Gi Number:
9978744
Ncbi Gene Id Number:
8431
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function.
Background Reference 1:
Zhou, T., et al. J. Biol. Chem. 285(32):24871-24881(2010) Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010) Kim, K., et al. Biochem. J. 427(3):413-422(2010) Yang, Z., et al. J. Int. Med. Res. 38(1):142-149(2010) Ondracek, C.R., et al. J. Virol. 83(23):12535-12544(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-606

List Size:
400 ul

List Price:

$495.00

Datasheet
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