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Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.

NR1H3 Antibody

CATALOG NUMBER: 25-443

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR1H3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NR1H3 antibody can be used for detection of NR1H3 by ELISA at 1:62500. NR1H3 antibody can be used for detection of NR1H3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1219 - MCF7 Cell Lysate
Predicted Molecular Weight:
50 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NR1H3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NR1H3
Additional Names:
NR1H3, LXR-a, LXRA, RLD-1
Protein Accession Number:
NP_005684
Protein Gi Number:
194294517
Ncbi Gene Id Number:
10062
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Interaction of NR1H3 with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. NR1H3 are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. NR1H3 Plays an important role in the regulation of cholesterol homeostasis.
Background Reference 1:
Torra, I.P., (2008) Mol. Cell. Biol. 28 (8), 2626-2636.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-443

List Size:
100 ul

List Price:

$519.00

Datasheet
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