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Western blot analysis in HepG2 cell line lysates (35ug/lane).
Western blot analysis in HepG2 cell line lysates (35ug/lane).

NR1H4 Antibody

CATALOG NUMBER: 60-257

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This NR1H4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-46 amino acids from the N-terminal region of human NR1H4.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
56 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NR1H4
Additional Names:
Bile acid receptor, Farnesoid X-activated receptor, Farnesol receptor HRR-1, Nuclear receptor subfamily 1 group H member 4, Retinoid X receptor-interacting protein 14, RXR-interacting protein 14, NR1H4, BAR, FXR, HRR1, RIP14
Protein Accession Number:
Q96RI1
Protein Gi Number:
46577705
Ncbi Gene Id Number:
9971
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NR1H4 is a Ligand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP). Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus.
Background Reference 1:
Meyer Zu Schwabedissen, H.E., et al. Hepatology 52(5):1797-1807(2010)
Background Reference 2:
Popescu, I.R., et al. FEBS Lett. 584(13):2845-2851(2010)
Background Reference 3:
Gadaleta, R.M., et al. Biochim. Biophys. Acta 1801(7):683-692(2010)
Background Reference 4:
Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-257

List Size:
400 ul

List Price:

$495.00

Datasheet
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