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46-930 (1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-930 (3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-930 (1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

NR1I2 Antibody

CATALOG NUMBER: 46-930

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: EQFAITLKSYIECNR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx. 48kDa band observed in lysates of cell line HepG2 (calculated MW of 49.8kDa according to NP_003880.3). Recommended concentration: 1-3ug/ml.An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_071285.1; NP_148934.1; NP_003880.3).
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NR1I2
Additional Names:
PXR, NR1I2, nuclear receptor subfamily 1, group I, member 2, BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, SAR, SXR, orphan nuclear receptor, pregnane X receptor, steroid and xenobiotic receptor
Protein Accession Number:
NP_003880.3,NP_071285.1,NP_148934.1
Protein Gi Number:
34398348
Ncbi Gene Id Number:
8856
Background Reference 1:
Thakur JK, Arthanari H, Yang F, Pan SJ, Fan X, Breger J, Frueh DP, Gulshan K, Li DK, Mylonakis E, Struhl K, Moye-Rowley WS, Cormack BP, Wagner G, Näär AM. A nuclear receptor-like pathway regulating multidrug resistance in fungi. Nature 2008 Apr 452 (7187): 604-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-930

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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