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Western blot analysis in Jurkat cell line lysates (35ug/lane).
Fluorescent confocal image of Hela cell stained with NR2C2 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR2C2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NR2C2 immunoreactivity is localized to Nucleus significantly.
Western blot analysis in Jurkat cell line lysates (35ug/lane).

NR2C2 Antibody

CATALOG NUMBER: 60-472

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This NR2C2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-466 amino acids from the C-terminal region of human NR2C2.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
65 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NR2C2
Additional Names:
Nuclear receptor subfamily 2 group C member 2, Orphan nuclear receptor TAK1, Orphan nuclear receptor TR4, Testicular receptor 4, NR2C2, TAK1, TR4
Protein Accession Number:
P49116
Protein Gi Number:
1351190
Ncbi Gene Id Number:
7182
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Members of the nuclear hormone receptor family, such asNR2C2, act as ligand-activated transcription factors. The proteinshave an N-terminal transactivation domain, a central DNA-bindingdomain with 2 zinc fingers, and a ligand-binding domain at the Cterminus. The activated receptor/ligand complex is translocated tothe nucleus where it binds to hormone response elements of targetgenes (Yoshikawa et al., 1996 [PubMed 8661150]).
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Srivastava, R., et al. J. Biol. Chem. 285(15):11100-11105(2010)
Background Reference 3:
Ear, T., et al. J. Immunol. 184(7):3897-3906(2010)
Background Reference 4:
Suzuki, S., et al. J. Biol. Chem. 285(7):4441-4446(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-472

List Size:
400 ul

List Price:

$495.00

Datasheet
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