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Western blot analysis of NR3C1 Antibody in A549 cell line lysates (35ug/lane)
NR3C1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of NR3C1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Western blot analysis of NR3C1 Antibody in A549 cell line lysates (35ug/lane)

NR3C1 Antibody

CATALOG NUMBER: 61-691

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NR3C1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 713-742 amino acids from the C-terminal region of human NR3C1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
86 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NR3C1
Additional Names:
Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1, NR3C1, GRL
Protein Accession Number:
P04150
Protein Gi Number:
121069
Ncbi Gene Id Number:
2908
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NR3C1 is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance.
Background Reference 1:
Geelhoed, M.J., et al. BMC Med. Genet. 11, 39 (2010)
Background Reference 2:
Szilagyi, K., et al. Neuro Endocrinol. Lett. 30(5):629-636(2009)
Background Reference 3:
Tian, S., et al. Biochem. J. 367 (PT 3), 907-911 (2002)
Background Reference 4:
Itoh, M., et al. Mol. Endocrinol. 16(10):2382-2392(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-691

List Size:
400 ul

List Price:

$495.00

Datasheet
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