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Western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the detected NR3C1 protein (arrow).
NR3C1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Fluorescent confocal image of Hela cell stained with NR3C1 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR3C1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NR3C1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
Western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the detected NR3C1 protein (arrow).

NR3C1 Antibody

CATALOG NUMBER: 61-939

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rb
Immunogen:
This NR3C1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-262 amino acids from the Central region of human NR3C1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
86 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NR3C1
Additional Names:
Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1, NR3C1, GRL
Protein Accession Number:
P04150
Protein Gi Number:
121069
Ncbi Gene Id Number:
2908
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NR3C1 is a receptor forglucocorticoids that can act as both a transcription factor and asa regulator of other transcription factors. This protein can alsobe found in heteromeric cytoplasmic complexes along with heat shockfactors and immunophilins. The protein is typically found in thecytoplasm until it binds a ligand, which induces transport into thenucleus. Mutations in this gene are a cause of glucocorticoidresistance, or cortisol, resistance. Alternate splicing, the use ofat least three different promoters, and alternate translationinitiation sites result in several transcript variants encoding thesame protein or different isoforms, but the full-length nature ofsome variants has not been determined.
Background Reference 1:
Bosker, F.J., et al. Mol. Psychiatry (2010)
Background Reference 2:
Inkster, B., et al. Neuroimage (2010)
Background Reference 3:
Geelhoed, M.J., et al. BMC Med. Genet. 11, 39 (2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-939

List Size:
400 ul

List Price:

$495.00

Datasheet
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