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Antibody used in IHC on Human Stomach at 4.0-8.0 ug/ml.
Antibody used in IHC on Human Liver at 4.0-8.0 ug/ml.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in IHC on Human Testis.
Antibody used in IHC on Human Stomach at 4.0-8.0 ug/ml.

NR4A1 Antibody

CATALOG NUMBER: 27-450

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR4A1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NR4A1 antibody can be used for detection of NR4A1 by ELISA at 1:312500. NR4A1 antibody can be used for detection of NR4A1 by western blot at 1.45 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
66 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NR4A1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NR4A1
Additional Names:
NR4A1, HMR, N10, TR3, NP10, GFRP1, NAK-1, NGFIB, NUR77
Protein Accession Number:
NP_775180
Protein Gi Number:
27894344
Ncbi Gene Id Number:
3164
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NR4A1 encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis.
Background Reference 1:
Lu, L., et al., (2004) J. Clin. Endocrinol. Metab. 89 (8), 4113-4118.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-450

List Size:
100 ul

List Price:

$473.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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