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Antibody used in WB on Human Daudi at 20 ug/ml.
Antibody used in WB on Human Daudi at 20 ug/ml.

NR5A1 Antibody

CATALOG NUMBER: 27-623

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR5A1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NR5A1 antibody can be used for detection of NR5A1 by ELISA at 1:62500. NR5A1 antibody can be used for detection of NR5A1 by western blot at 20 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Predicted Molecular Weight:
52 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NR5A1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NR5A1
Additional Names:
NR5A1, ELP, SF1, FTZ1, POF7, SF-1, AD4BP, FTZF1, SPGF8, SRXY3
Protein Accession Number:
NP_004950
Protein Gi Number:
20070193
Ncbi Gene Id Number:
2516
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NR5A1 is a members of the orphan nuclear receptor superfamily that is critical regulatory components of the hypothalamic-pituitary-adrenal-gonadal axis. In adrenal and gonadal tissues they regulate the expression of the cytochrome P450 steroid hydroxylase genes, key mediators of steroidogenesis.
Background Reference 1:
Li, L.A., et al., (2004) J. Steroid Biochem. Mol. Biol. 91 (1-2), 11--20.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-623

List Size:
100 ul

List Price:

$473.00

Datasheet
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