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Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Antibody used in WB on Human HepG2 at 2.5 ug/ml.

NR5A2 Antibody

CATALOG NUMBER: 28-855

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR5A2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NR5A2 antibody can be used for detection of NR5A2 by ELISA at 1:12500. NR5A2 antibody can be used for detection of NR5A2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
61 kDa, 56 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NR5A2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NR5A2
Additional Names:
NR5A2, B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, hB1F, hB1F-2, LRH1
Protein Accession Number:
NP_995582
Protein Gi Number:
45545405
Ncbi Gene Id Number:
2494
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NR5A2 binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. It may be responsable for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. It is a key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. It may also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.
Background Reference 1:
Lee, Y.K., (2006) J. Biol. Chem. 281 (12), 7850-7855.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-855

List Size:
100 ul

List Price:

$473.00

Datasheet
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