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Western blot analysis in K562 cell line lysates (35ug/lane).
Western blot analysis in K562 cell line lysates (35ug/lane).

NRF1 Antibody

CATALOG NUMBER: 57-796

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Mouse
Immunogen:
This NRF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-255 amino acids from the Central region of human NRF1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NRF1
Additional Names:
Nuclear respiratory factor 1, NRF-1, Alpha palindromic-binding protein, Alpha-pal, NRF1
Protein Accession Number:
Q16656
Protein Gi Number:
12643732
Ncbi Gene Id Number:
4899
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that homodimerizes andfunctions as a transcription factor which activates the expressionof some key metabolic genes regulating cellular growth and nucleargenes required for respiration, heme biosynthesis, andmitochondrial DNA transcription and replication. The protein hasalso been associated with the regulation of neurite outgrowth.Alternate transcriptional splice variants, which encode the sameprotein, have been characterized. Additional variants encodingdifferent protein isoforms have been described but they have notbeen fully characterized. Confusion has occurred in bibliographicdatabases due to the shared symbol of NRF1 for this gene and for'nuclear factor (erythroid-derived 2)-like 1' which has an officialsymbol of NFE2L1.
Background Reference 1:
Gonen, N., et al. J. Biol. Chem. 285(44):33602-33613(2010)
Background Reference 2:
Zhang, Y., et al. Biochem. J. 430(3):497-510(2010)
Background Reference 3:
Shao, D., et al. Mitochondrion 10(5):516-527(2010)
Background Reference 4:
Adam, T., et al. Biochem. Biophys. Res. Commun. 398(3):495-499(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-796

List Size:
400 ul

List Price:

$495.00

Datasheet
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