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Western blot analysis of NSA1 in rat liver tissue lysate with NSA1 antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of NSA1 in rat liver tissue lysate with NSA1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

NSA1 Antibody

CATALOG NUMBER: 5671

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (79%)
Immunogen:
NSA1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human NSA1.
The immunogen is located within amino acids 90 - 140 of NSA1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NSA1 antibody can be used for detection of NSA1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Purification:
NSA1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NSA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NSA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WDR74
Additional Names:
NSA1 Antibody: NSA1, WD repeat-containing protein 74, NOP seven-associated protein 1
Protein Accession Number:
Q6RFH5
Protein Gi Number:
55976441
Ncbi Gene Id Number:
54663
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NSA1 Antibody: The yeast nucleolar protein NOP7 is necessary for the maturation of 66S preribosomes and interacts with numerous other proteins. One such protein is an essential, conserved WD repeat protein, NOP seven-associated protein 1 (NSA1), that is also required for the yeast 66S ribosome assembly. NSA1 is also associated with the AAA ATPase Rix7, and release of NSA1 from a novel late nucleolar pre-60S requires the Rix7 function. NSA1 has also been found upregulated in mammalian cancer cells, suggesting it may also play a role in cell proliferation.
Background Reference 1:
Miles TD, Jakovljevic J, Horsey EW, et al. Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes. Mol. Cell Biol.2005; 25:10419-32.
Background Reference 2:
Harnpicharnchai P, Jakovljevic J, Horsey E, et al. Composition and functional characterization of yeast 66S ribosome assembly intermediates. Mol. Cell2001; 8:505-15.
Background Reference 3:
Kressler D, Roser D, Pertschy B, et al. The AAA ATPase Rix7 powers progression of ribosome biogenesis by stripping Nsa1 from pre-60S particles. J. Cell Biol.2008; 181:935-44.
Background Reference 4:
Krol M, Polanska J, Pawlowski KM, et al. Transcriptomic signature of cell lines isolated from canine mammary adenocarcinoma metastases to lungs. J. Appl. Genet.2010; 51:37-50.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5671

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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