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Antibody used in WB on Human HepG2 cells at 2.5 ug/ml.
Antibody used in WB on Human HepG2 cells at 2.5 ug/ml.

NSDHL Antibody

CATALOG NUMBER: 30-269

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NSDHL.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NSDHL antibody can be used for detection of NSDHL by ELISA at 1:312500. NSDHL antibody can be used for detection of NSDHL by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
42 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NSDHL antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NSDHL
Additional Names:
NSDHL, H105E3, XAP104, SDR31E1
Protein Accession Number:
NP_057006
Protein Gi Number:
8393516
Ncbi Gene Id Number:
50814
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NSDHL is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in NSDHL gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males.The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene.
Background Reference 1:
Guggenberger, C., J. Steroid Biochem. Mol. Biol. 104 (3-5), 105-109 (2007).

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-269

List Size:
100 ul

List Price:

$473.00

Datasheet
Shipping Info

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