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Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).
NSMAF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).

NSMAF Antibody

CATALOG NUMBER: 57-140

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
This NSMAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-57 amino acids from the N-terminal region of human NSMAF.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
104 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NSMAF
Additional Names:
Protein FAN, Factor associated with neutral sphingomyelinase activation, Factor associated with N-SMase activation, NSMAF, FAN
Protein Accession Number:
Q92636
Protein Gi Number:
209572614
Ncbi Gene Id Number:
8439
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a WD-repeat protein that binds thecytoplasmic sphingomyelinase activation domain of the 55kD tumornecrosis factor receptor. This protein is required for TNF-mediatedactivation of neutral sphingomyelinase and may play a role inregulating TNF-induced cellular responses such as inflammation.Alternative splicing results in multiple transcript variants.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care (2010) In press :
Background Reference 2:
Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009)
Background Reference 3:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Background Reference 4:
Clarke, C.J., et al. Mol. Pharmacol. 74(4):1022-1032(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-140

List Size:
400 ul

List Price:

$495.00

Datasheet
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