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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse spleen tissue lysates (35ug/lane).
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.

NSUN2 Antibody

CATALOG NUMBER: 55-438

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This NSUN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423-451 amino acids from the Central region of human NSUN2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
86 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NSUN2
Additional Names:
tRNA (cytosine(34)-C(5))-methyltransferase, Myc-induced SUN domain-containing protein, Misu, NOL1/NOP2/Sun domain family member 2, Substrate of AIM1/Aurora kinase B, tRNA (cytosine-5-)-methyltransferase, tRNA methyltransferase 4 homolog, hTrm4, NSUN2, SAKI, TRM4
Protein Accession Number:
Q08J23
Protein Gi Number:
148887180
Ncbi Gene Id Number:
54888
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Maturation of cytoplasmic tRNAs includes splicing ofintrons, which are located 1 nucleotide 3-prime from the anticodonin all intron-containing tRNA genes. In tRNA-leu(CAA), the firstposition of the anticodon, C34, is converted to 5-methylcytosine, amodification necessary to stabilize the anticodon-codon pairing andcorrectly translate the mRNA. NSUN2 encodes a methyltransferasethat catalyzes the intron-dependent formation of 5-methylcytosineat C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed17071714]).
Background Reference 1:
Bailey, S.D., et al. Diabetes Care (2010) In press :
Background Reference 2:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 3:
Frye, M., et al. Cancer Lett. 289(1):71-80(2010)
Background Reference 4:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-438

List Size:
400 ul

List Price:

$495.00

Datasheet
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