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Western blot analysis of extracts of various cell lines, using NSUN6 antibody (22-782) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 40s.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug NSUN6 antibody (22-782). Western blot was performed from the immunoprecipitate using NSUN6 antibody (22-782) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using NSUN6 antibody (22-782) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 40s.

NSUN6 Antibody

CATALOG NUMBER: 22-782

Clonality:
Polyclonal
Tested Applications:
IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 220-469 of human NSUN6 (NP_872349.1).
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB: 1:500 - 1:2000
IP: 1:50 - 1:200
Positive Control 1:
Jurkat
Positive Control 2:
HepG2
Positive Control 3:
HeLa
Positive Control 4:
293T
Positive Control 5:
Mouse testis
Predicted Molecular Weight:
Observed: 52kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NSUN6
Additional Names:
NSUN6, 4933414E04Rik, FLJ23743, NOPD1
Ncbi Gene Id Number:
221078
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-782

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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