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Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: RPMI-8226 whole cell lysate Lane 3: SH-SY5Y lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: RPMI-8226 whole cell lysate Lane 3: SH-SY5Y lysate Lysates/proteins at 20 ug per lane.

NT5M Antibody

CATALOG NUMBER: 61-197

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This NT5M antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 195-226 amino acids from human NT5M.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
26 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NT5M
Additional Names:
5'(3')-deoxyribonucleotidase, mitochondrial, 5',3'-nucleotidase, mitochondrial, 3.1.3.-, Deoxy-5'-nucleotidase 2, dNT-2, NT5M, DNT2
Protein Accession Number:
Q9NPB1
Protein Gi Number:
38258255
Ncbi Gene Id Number:
56953
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Dephosphorylates specifically the 5' and 2'(3')- phosphates of uracil and thymine deoxyribonucleotides, and so protects mitochondrial DNA replication from excess dTTP. Has only marginal activity towards dIMP and dGMP.
Background Reference 1:
Rampazzo C.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:8239-8244(2000).
Background Reference 2:
Rinaldo-Matthis A.,et al.Nat. Struct. Biol. 9:779-787(2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-197

List Size:
200 ul

List Price:

$495.00

Datasheet
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