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Western Blot at 1:1000 dilution Lane 1: DU145 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Hela,NCI-H460 cell line lysates (35ug/lane).This demonstrates the NTHL1 antibody detected the NTHL1 protein (arrow).
Western Blot at 1:1000 dilution Lane 1: DU145 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.

NTHL1 Antibody

CATALOG NUMBER: 55-909

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NTHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human NTHL1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
34 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NTHL1
Additional Names:
Endonuclease III-like protein 1 {ECO:0000255, HAMAP-Rule:MF_03183}, hNTH1, 322- {ECO:0000255, HAMAP-Rule:MF_03183}, 429918 {ECO:0000255, HAMAP-Rule:MF_03183}, Bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyase {ECO:0000255, HAMAP-Rule:MF_03183}, DNA glycoslyase/AP lyase {ECO:0000255, HAMAP-Rule:MF_03183}, NTHL1 {ECO:0000255, HAMAP-Rule:MF_03183}, NTH1, OCTS3
Protein Accession Number:
P78549
Protein Gi Number:
29840795
Ncbi Gene Id Number:
4913
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a DNA N-glycosylase ofthe endonuclease III family. Like a similar protein in E. coli, theencoded protein has DNA glycosylase activity on DNA substratescontaining oxidized pyrimidine residues and hasapurinic/apyrimidinic lyase activity.
Background Reference 1:
Wang, W., et al. Nucleic Acids Res. (2010) In press :
Background Reference 2:
Arora, M., et al. Leukemia 24(8):1470-1475(2010)
Background Reference 3:
Thyagarajan, B., et al. Biol. Blood Marrow Transplant. 16(8):1084-1089(2010)
Background Reference 4:
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-909

List Size:
400 ul

List Price:

$495.00

Datasheet
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