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Western blot analysis of extracts of various cell lines, using NTRK1 antibody (18-498) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse stomach using NTRK1 Antibody (18-498) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat cerebellum using NTRK1 Antibody (18-498) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using TRKA antibody (18-498) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using NTRK1 antibody (18-498) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

NTRK1 Antibody

CATALOG NUMBER: 18-498

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NTRK1 (NP_002520.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
K-562
Positive Control 3:
NIH/3T3
Positive Control 4:
Mouse liver
Positive Control 5:
Mouse heart
Positive Control 6:
Rat liver
Predicted Molecular Weight:
Observed: 110kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NTRK1
Additional Names:
NTRK1, Oncogene TRK, TrkA/NGF receptor, Tropomyosin-related kinase A, TRK, TRK1, Tyrosine kinase receptor, Tyrosine kinase receptor A, gp140trk, MTC, p140-TrkA, Trk-A, TRKA
Ncbi Gene Id Number:
4914
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-498

List Size:
50 uL

List Price:

$309.00

Out of stock

Datasheet
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