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Western blot analysis of extracts of HepG2 cells, using NUBP1 antibody (16-590) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat heart using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of HepG2 cells, using NUBP1 antibody (16-590) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

NUBP1 Antibody

CATALOG NUMBER: 16-590

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 201-320 of human NUBP1 (NP_002475.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HepG2
Predicted Molecular Weight:
Observed: 38kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NUBP1
Additional Names:
Cytosolic Fe-S cluster assembly factor NUBP1 {ECO:0000255, HAMAP-Rule:MF_03038}, Nucleotide-binding protein 1 {ECO:0000255, HAMAP-Rule:MF_03038}, NBP 1 {ECO:0000255, HAMAP-Rule:MF_03038}, NUBP1 {ECO:0000255, HAMAP-Rule:MF_03038}, NBP, NBP1
Ncbi Gene Id Number:
4682
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NUBP1 is a member of the NUBP/MRP subfamily of ATP-binding proteins.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-590

List Size:
100 uL

List Price:

$436.00

Datasheet
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