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Western blot analysis of Nucleobindin-2 in rat brain tissue lysate with Nucleobindin-2 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of Nucleobindin-2 in rat brain tissue with Nucleobindin-2 antibody at 2.5 μg/ml.
Immunofluorescence of Nucleobindin-2 in rat brain tissue with Nucleobindin-2 antibody at 20 μg/ml.
Western blot analysis of Nucleobindin-2 in rat brain tissue lysate with Nucleobindin-2 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

Nucleobindin-2 Antibody

CATALOG NUMBER: 7271

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
Nucleobindin-2 antibody was raised against a 16 amino acid peptide near the center of human Nucleobindin-2 .
The immunogen is located within amino acids 260 - 310 of Nucleobindin-2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Nucleobindin-2 Antibody can be used for detection of Nucleobindin-2 by Western blot starting at 0.5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
Nucleobindin-2 antibody is predicted to not cross-react with NUCB1.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 46 kDa
Observed: 54 kDa
Purification:
Nucleobindin-2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Nucleobindin-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Nucleobindin-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
NUCB2
Additional Names:
Nucleobindin-2 Antibody: NEFA, HEL-S-109, NEFA, Nucleobindin-2, DNA-binding protein NEFA
Protein Accession Number:
NP_005004
Protein Gi Number:
4826870
Ncbi Gene Id Number:
4925
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Nucleobindin-2 Antibody: NUCB2 was initially identified as calcium-binding protein EF hand motif-containing protein that could bind with Necdin, a growth suppressor expressed primarily in postmitotic neurons. NUC2 is a secreted protein that is cleaved into three major peptide products, Nesfatin-1, Nesfatin-2, and Nesfatin-3, of which Nesfatin-1 has been found to cause the suppression of food intake in a leptin-independent manner. Other studies have suggested that NUCB2/Nesfatin-1 may also play roles in energy homeostasis and closely related neuroendocrine functions.
Background Reference 1:
Taniguchi N, Taniura H, Niinobe M, et al. The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm. J. Biol. Chem. 2000; 275:31674-85.
Background Reference 2:
Oh IS, Shimizu H, Satoh T, et al. Identification of nesfatin-1 as a satiety molecule in the hypothalamus. Nature 2006; 443:709-12.
Background Reference 3:
Garcia-Galiano D, Navarro VM, Gaytan F, et al. Expanding roles of NUCB2/nesfatin-1 in neuroendocrine regulation. J. Mol. Endocrin. 2010; 45:281-90.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7271

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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