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Western blot analysis of Nudel in Jurkat whole cell lysate with Nudel antibody at (A) 0.5, (B) 1, or (C) 2 μg/mL.
Immunocytochemistry of Nudel in Jurkat cells with Nudel antibody at 2 μg/mL.
Immunofluorescence of Nudel in Jurkat cells with Nudel antibody at 10 μg/mL.
Western blot analysis of Nudel in Jurkat whole cell lysate with Nudel antibody at (A) 0.5, (B) 1, or (C) 2 μg/mL.
Green Product
Trial Size

Nudel Antibody

CATALOG NUMBER: 3009

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Rabbit: (100%), Chicken: (73%)
Immunogen:
Nudel antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Nudel.
The immunogen is located within the first 50 amino acids of Nudel.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
Nudel antibody can be used for detection of Nudel by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2:
Cat. No. 17-005 - Jurkat Cell Slide
Purification:
Nudel Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Nudel Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Nudel antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NDEL1
Additional Names:
Nudel Antibody: EOPA, NDE2, NUDEL, MITAP1, NDE1L1, EOPA, Nuclear distribution protein nudE-like 1, Mitosin-associated protein 1, Protein Nudel
Protein Accession Number:
AAF97497
Protein Gi Number:
12043569
Ncbi Gene Id Number:
81565
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Nudel Antibody: Nudel was initially discovered as a protein homologous to Aspergillus NUDE and that associated with Lis1 and polyprotein complex cytoplasmic dynein, both of which have been linked to neuronal development and migration. It was later shown to be a substrate of cdk5, a kinase known to be critical during neuronal migration; phosphorylation of Nudel by cdk5 affects its localization in neurons and affects neuritic morphology. It is thought that together with Lis1, Nudel regulates cytoplasmic dynein, a large polyprotein complex, in neuronal migration and mitosis through direct interactions. Similar interactions are thought to also play a role in membrane traffic in other cells as disruption of Nudel expression through RNA interference perturbed normal endomembrane flux and resulted in the fragmentation of the Golgi apparatus.
Background Reference 1:
Niethammer H, Smith DS, Ayala R, et al. NUDEL is a novel Cdk5 substrate that associates with LIS1 and cytoplasmic dynein. Neuron 2000; 28:697-711.
Background Reference 2:
Liu Z, Steward R, and Luo L. Drosophila Lis1 is required for neuroblast proliferation, dendritic elaboration and axonal transport. Nat. Cell Biol. 2000; 2:776-83.
Background Reference 3:
Gupta A and Tsai LH. Cyclin-dependent kinase 5 and neuronal migration in the neocortex. Neurosignals 2003; 12:173-9.
Background Reference 4:
Liang Y, Yu W, Li Y, et al. Nudel functions in membrane traffic mainly through association with Lis1 and cytoplasmic dynein. J. Cell Biol. 2004; 164:557-66.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3009

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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