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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human Numbl with C-terminal MYC tag probed with 43-109 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-109 (1mg/ml) in Lane B. Primary
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human Numbl with C-terminal MYC tag probed with 43-109 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-109 (1mg/ml) in Lane B. Primary

NUMBL Antibody

CATALOG NUMBER: 43-109

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: SRSAAASGGPRRPE-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:In transfected HEK293 transiently expressing Human Numbl and DYKDDDDK tagged), a band of approx. 85kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.5-1ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NUMBL
Additional Names:
NUMBL, numb homolog (Drosophila)-like, NBL, CAG3A, CTG3a, NUMBR, NUMB-R, TNRC23, numb (Drosophila) homolog-like, NUMBLIKE, TNRC23, numb homolog-like
Protein Accession Number:
NP_004747.1
Protein Gi Number:
10863899
Ncbi Gene Id Number:
9253
Background Reference 1:
Petersen PH, Zou K, Hwang JK, Jan YN, Zhong W.Progenitor cell maintenance requires numb and numblike during mouse neurogenesis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-109

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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