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Western blot analysis of NUP155 in P815 cell lysate with NUP155 antibody at (A) 0.5 and (B) 1 μg/mL.
Western blot analysis of NUP155 in P815 cell lysate with NUP155 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

NUP155 Antibody

CATALOG NUMBER: 4703

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%)
Immunogen:
NUP155 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human NUP155.
The immunogen is located within the last 50 amino acids of NUP155.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NUP155 antibody can be used for detection of NUP155 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1286 - P815 Cell Lysate
Purification:
NUP155 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NUP155 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NUP155 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NUP155
Additional Names:
NUP155 Antibody: N155, KIAA0791, Nuclear pore complex protein Nup155, 155 kDa nucleoporin
Protein Accession Number:
NP_705618
Protein Gi Number:
24430149
Ncbi Gene Id Number:
9631
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NUP155 Antibody: The nuclear pore complex (NPC) is a protein assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. One protein in this assembly is the nucleoporin (NUP)-155, which is localized symmetrically to both the nucleoplasmic and cytoplasmic faces of the NPC. NUP155 has been reported to interact with both NUP35 and the mRNA export factor Gle1, but the functions of these interactions are still unknown. NUP155 has also been shown to be required for NPC assembly and nuclear envelope (NE) membrane fusion. NUP155 is recruited late in NE formation, suggesting that NUP155 defines an essential late step in NE assembly. NUP155 has recently been identified as an HIV dependency factor (HDF), suggesting that NUP155 may be an important drug target in HIV treatment. At least two isoforms of NUP155 are known to exist.
Background Reference 1:
Tran EJ and Wente SR. Dynamic nuclear pore complex: life on the edge. Cell2006; 125:1041-53.
Background Reference 2:
Radu A, Blobel G and Wozniak RW. Nup155 is a novel nuclear pore complex protein that contains neither repetitive sequence motifs nor reacts with WGA. J. Cell Biol.1993; 121:1-9.
Background Reference 3:
Hawryluk-Gara LA, Shibuya EK, and Wozniak RW. Vertebrate Nup53 interacts with the nuclear lamina and is required for the assembly of a Nup93-containing complex. Mol. Biol. Cell2005; 16:2382-94.
Background Reference 4:
Rayala HJ, Kendirgi F, Barry DM, et al. The mRNA export factor Gle1 interacts with the nuclear pore complex protein Nup155. Mol. Cell Proteomics2004; 3:145-55.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4703

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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