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Western blot analysis of NUP210 Antibody in T47D cell line lysates (35ug/lane)
NUP210 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of NUP210 Antibody in T47D cell line lysates (35ug/lane)

NUP210 Antibody

CATALOG NUMBER: 64-209

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This NUP210 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202-231 amino acids from the N-terminal region of human NUP210.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
205 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NUP210
Additional Names:
Nuclear pore membrane glycoprotein 210, Nuclear pore protein gp210, Nuclear envelope pore membrane protein POM 210, POM210, Nucleoporin Nup210, Pore membrane protein of 210 kDa, NUP210, KIAA0906
Protein Accession Number:
Q8TEM1
Protein Gi Number:
116242720
Ncbi Gene Id Number:
23225
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NUP210 nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a membrane-spanning glycoprotein that is a major component of the nuclear pore complex.
Background Reference 1:
Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
Background Reference 2:
Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
Background Reference 3:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 4:
Stavru, F., et al. J. Cell Biol. 173(4):477-483(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-209

List Size:
400 ul

List Price:

$495.00

Datasheet
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