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Western blot analysis in Hela cell line lysates (35ug/lane).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Hela cell line lysates (35ug/lane).

NUP35 Antibody

CATALOG NUMBER: 56-703

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NUP35 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 289-318 amino acids from the C-terminal region of human NUP35.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
35 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NUP35
Additional Names:
Nucleoporin NUP53, 35 kDa nucleoporin, Mitotic phosphoprotein 44, MP-44, Nuclear pore complex protein Nup53, Nucleoporin Nup35, NUP35, MP44, NUP53
Protein Accession Number:
Q8NFH5
Protein Gi Number:
74730292
Ncbi Gene Id Number:
129401
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the nucleoporin family. Theprotein is localized to the nuclear rim and is part of the nuclearpore complex (NPC). All molecules entering or leaving the nucleuseither diffuse through or are actively transported by the NPC.
Background Reference 1:
Ichikawa, S., et al. J. Bone Miner. Res. 25(8):1821-1829(2010)
Background Reference 2:
Hawryluk-Gara, L.A., et al. Mol. Biol. Cell 19(4):1753-1762(2008)
Background Reference 3:
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Background Reference 4:
Lamesch, P., et al. Genomics 89(3):307-315(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-703

List Size:
400 ul

List Price:

$495.00

Datasheet
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