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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in IF on Human HeLa.
Antibody used in WB on Human HepG2 at 1 ug/ml.
Antibody used in WB on Human Hela at 1 ug/ml.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

NUP50 Antibody

CATALOG NUMBER: 30-804

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NUP50.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NUP50 antibody can be used for detection of NUP50 by ELISA at 1:312500. NUP50 antibody can be used for detection of NUP50 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
50 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NUP50 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NUP50
Additional Names:
NUP50, MGC39961, NPAP60, NPAP60L
Protein Accession Number:
NP_009103
Protein Gi Number:
24497451
Ncbi Gene Id Number:
10762
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import.The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.
Background Reference 1:
Olsen, J.V., (2006) Cell 127 (3), 635-648.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-804

List Size:
100 ul

List Price:

$519.00

Datasheet
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