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Western blot analysis in WiDr cell line lysates (35ug/lane).
Western blot analysis in WiDr cell line lysates (35ug/lane).

NUP50 Antibody

CATALOG NUMBER: 58-676

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NUP50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-252 amino acids from the Central region of human NUP50.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NUP50
Additional Names:
Nuclear pore complex protein Nup50, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, Nucleoporin Nup50, NUP50, NPAP60L
Protein Accession Number:
Q9UKX7
Protein Gi Number:
20455193
Ncbi Gene Id Number:
10762
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The nuclear pore complex is a massive structure thatextends across the nuclear envelope, forming a gateway thatregulates the flow of macromolecules between the nucleus and thecytoplasm. Nucleoporins are the main components of the nuclear porecomplex in eukaryotic cells. The protein encoded by this gene is amember of the FG-repeat containing nucleoporins that functions as asoluble cofactor in importin-alpha:beta-mediated nuclear proteinimport. Pseudogenes of this gene are found on chromosomes 5, 6, and14. Two transcript variants encoding different isoforms have beenfound for this gene.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Ogawa, Y., et al. Mol. Biol. Cell 21(4):630-638(2010)
Background Reference 3:
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Background Reference 4:
Olsen, J.V., et al. Cell 127(3):635-648(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-676

List Size:
400 ul

List Price:

$495.00

Datasheet
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