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Western blot analysis of NUP50 using rabbit polyclonal NUP50 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUP50 gene (Lane 2).
Western blot analysis of anti-NUP50 Pab in mouse brain tissue lysate.
Western blot analysis of NUP50 using rabbit polyclonal NUP50 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUP50 gene (Lane 2).

NUP50 Antibody

CATALOG NUMBER: 60-337

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This NUP50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 432-461 amino acids from the C-terminal region of human NUP50.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NUP50
Additional Names:
Nuclear pore complex protein Nup50, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, Nucleoporin Nup50, NUP50, NPAP60L
Protein Accession Number:
Q9UKX7
Protein Gi Number:
20455193
Ncbi Gene Id Number:
10762
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. NUP50 may serve as a binding site on the nuclear side of the pore complex for export receptor-cargo complexes. It interacts with multiple transport receptor proteins including p27Kip1. This interaction is required for correct intracellular transport and degradation of p27Kip1.
Background Reference 1:
Lindsay, M.E., et al., Cell 110(3):349-360 (2002).
Background Reference 2:
Swaminathan, S., et al., Dev. Cell 3(3):304-306 (2002).
Background Reference 3:
Smitherman, M., et al., Mol. Cell. Biol. 20(15):5631-5642 (2000).
Background Reference 4:
Guan, T., et al., Mol. Cell. Biol. 20(15):5619-5630 (2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-337

List Size:
400 ul

List Price:

$495.00

Datasheet
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