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Western blot analysis of in HepG2 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of MDA-231 cells using (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of in HepG2 cell line lysates (35ug/lane)

NUP62 Antibody

CATALOG NUMBER: 62-947

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NUP62 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 492-520 amino acids from the C-terminal region of human NUP62.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
53 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NUP62
Additional Names:
Nuclear pore glycoprotein p62, 62 kDa nucleoporin, Nucleoporin Nup62, NUP62
Protein Accession Number:
P37198
Protein Gi Number:
134047855
Ncbi Gene Id Number:
23636
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NUP62 is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This protein is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. The protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals.
Background Reference 1:
Stochaj,U., Banski,P. Exp. Cell Res. 312 (13), 2490-2499 (2006)
Background Reference 2:
Basel-Vanagaite,L., Muncher,L. Ann. Neurol. 60 (2), 214-222 (2006)
Background Reference 3:
Guan,T., Muller,S. Mol. Biol. Cell(11), 1591-1603 (1995)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-947

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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