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45-102 (1ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-102 (1ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

NUP85 Antibody

CATALOG NUMBER: 45-102

Clonality:
Polyclonal
Tested Applications:
ELISA, IP, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-ARAIIREGSLEGS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in HeLa lysates (calculated MW of 75kDa according to NP_079120). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human: Toda E et al, J Immunol. 2009 Nov 15;183(10):6387-94. PMID: 19841162.Immunoprecipitation: This antibody was successfully used in IP on Human: Toda E et al, J Immunol. 2009 Nov 15;183(10):6387-94. PMID: 19841162.
Specificity:
Please note that, in contrast to other anti-Pericentrin antibodies available, this ab is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75KDa band (the expected size according to NP_079120) and not a 220kDa band (as reported for other anti-pericentrin antibodies).
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NUP85
Additional Names:
NUP85, pericentrin 1, PCNT1, PCN, PCNT, frount, nucleoporin Nup75, nucleoporin 85kDa, FLJ12549, Nup75, nucleoporin 85
Protein Accession Number:
NP_079120.1
Protein Gi Number:
13376259
Ncbi Gene Id Number:
79902
Background Reference 1:
Doxsey SJ, Stein P, Evans L, Calarco PD, Kirschner M. Pericentrin, a highly conserved centrosome protein involved in microtubule organization. Cell. 1994 Feb 25;76(4):639-50.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-102

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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