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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MOLT-4 whole cell lysate Lane 5: NCI-H1299 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MOLT-4 whole cell lysate Lane 5: NCI-H1299 whole cell lysate Lysates/proteins at 20 ug per lane.

NUP85 Antibody

CATALOG NUMBER: 61-273

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NUP85 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 163-195 amino acids from human NUP85.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
75 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NUP85
Additional Names:
Nuclear pore complex protein Nup85, 85 kDa nucleoporin, FROUNT, Nucleoporin Nup75, Nucleoporin Nup85, Pericentrin-1, NUP85, NUP75, PCNT1
Protein Accession Number:
Q9BW27
Protein Gi Number:
74733394
Ncbi Gene Id Number:
79902
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2- activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl- inositol 3-kinase-Rac-lammellipodium protrusion cascade.
Background Reference 1:
Cronshaw J.M.,et al.J. Cell Biol. 158:915-927(2002).
Background Reference 2:
Terashima Y.,et al.Nat. Immunol. 6:827-835(2005).
Background Reference 3:
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Background Reference 4:
Bechtel S.,et al.BMC Genomics 8:399-399(2007).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-273

List Size:
200 ul

List Price:

$495.00

Datasheet
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