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45-988 (2ug/ml) staining of Human Thyroid lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-988 (5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-988 (2ug/ml) staining of Human Thyroid lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Nur77 Antibody

CATALOG NUMBER: 45-988

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-GRLPSKPKQPPDAS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in Human Thyroid gland lysates (calculated MW of 64.5kDa according to NP_002126.2 and NP_775180.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Breast. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognise isoform a (NP_002126.2 and NP_775180.1) only.
Positive Control 1:
Cat. No. 1380 - Human Thyroid Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NR4A1
Additional Names:
NR4A1, NUR77, nuclear receptor subfamily 4, group A, member 1 , GFRP1, HMR, MGC9485, N10, NAK-1, NGFIB, NP10, TR3 , TR3 orphan receptor, early response protein NAK1, growth factor-inducible nuclear protein N10, hormone receptor, orphan nuclear receptor HMR, steroid receptor TR3
Protein Accession Number:
NP_002126.2,NP_775180.1
Protein Gi Number:
21361342
Ncbi Gene Id Number:
3164
Background Reference 1:
Kiss I, Oskolas H, Toth R, Bouillet P, Toth K, Fulop A, Scholtz B, Ledent C, Fesus L, Szondy Z. Adenosine A(2A )receptor-mediated cell death of mouse thymocytes involves adenylate cyclase and Bim and is negatively regulated by Nur77. Eur J Immunol. 2006 Jun;36(6):1559-71.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-988

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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