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Western blot analysis in U251 cell line lysates (35ug/lane).
NUSAP antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in U251 cell line lysates (35ug/lane).

NUSAP Antibody

CATALOG NUMBER: 55-549

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NUSAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 amino acids from the N-terminal region of human NUSAP.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
49 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NUSAP1
Additional Names:
Nucleolar and spindle-associated protein 1, NuSAP, NUSAP1, ANKT
Protein Accession Number:
Q9BXS6
Protein Gi Number:
74717631
Ncbi Gene Id Number:
51203
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NUSAP1 is a nucleolar-spindle-associated protein thatplays a role in spindle microtubule organization (Raemaekers etal., 2003 [PubMed 12963707]).
Background Reference 1:
Wadia, P.P., et al. Blood 115(10):2077-2087(2010)
Background Reference 2:
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Background Reference 3:
Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
Background Reference 4:
Raemaekers, T., et al. J. Cell Biol. 162(6):1017-1029(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-549

List Size:
400 ul

List Price:

$495.00

Datasheet
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