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Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.
Antibody used in WB on Human Jurkat 2.5 ug/ml.
Antibody used in WB on Human HeLa at: 1:100 (Lane 1: 7ug HeLa cystosolic extract, Lane 2: 7ug HeLa nuclear extract).
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.

NXF5 Antibody

CATALOG NUMBER: 29-483

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NXF5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
NXF5 antibody can be used for detection of NXF5 by ELISA at 1:312500. NXF5 antibody can be used for detection of NXF5 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
44 kDa, 72 kDa, 72 kDa, 72 kDa, 72 kDa, 72 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NXF5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NXF5
Additional Names:
NXF5, TAPL1
Protein Accession Number:
NP_116564
Protein Gi Number:
254039592
Ncbi Gene Id Number:
55998
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NXF5 is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity.This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. Five transcript variants that encode different isoforms have been found for this gene.
Background Reference 1:
Frints, S.G., (2003) Am. J. Med. Genet. A 119 (3), 367-374.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-483

List Size:
100 ul

List Price:

$473.00

Datasheet
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