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Western blot analysis in mouse cerebellum,brain tissue lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human brain tissue reacted with NXPH1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse cerebellum,brain tissue lysates (35ug/lane).

NXPH1 Antibody

CATALOG NUMBER: 63-997

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This NXPH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from the N-terminal region of human NXPH1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
31 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NXPH1
Additional Names:
Neurexophilin-1, NXPH1, NPH1
Protein Accession Number:
P58417
Protein Gi Number:
17433155
Ncbi Gene Id Number:
30010
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NXPH1 is a secreted protein with a variable N-terminal domain, a highly conserved, N-glycosylated central domain, a short linker region, and a cysteine-rich C-terminal domain. This protein forms a very tight complex with alpha neurexins, a group of proteins that promote adhesion between dendrites and axons.
Background Reference 1:
Xu, J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(5):2136-2140(2010)
Background Reference 2:
Shen, L., et al. Neuroimage (2010) In press :
Background Reference 3:
van den Oord, E.J., et al. Arch. Gen. Psychiatry 65(9):1062-1071(2008)
Background Reference 4:
Melzer, D., et al. PLoS Genet.(5), E1000072 (2008) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-997

List Size:
400 ul

List Price:

$495.00

Datasheet
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