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Antibody is used in Western blot to detect NYREN18 in 293 cell lysate.
Western blot analysis of NUB1 using NYREN18 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUB1 gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Antibody is used in Western blot to detect NYREN18 in 293 cell lysate.

NYREN18 Antibody

CATALOG NUMBER: 61-405

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NYREN18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-542 amino acids from the C-terminal region of human NYREN18.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
71 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NUB1
Additional Names:
NEDD8 ultimate buster 1, Negative regulator of ubiquitin-like proteins 1, Renal carcinoma antigen NY-REN-18, NUB1, NYREN18
Protein Accession Number:
Q9Y5A7
Protein Gi Number:
37154888
Ncbi Gene Id Number:
51667
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NYREN18 is a specific down-regulator of the NEDD8 conjugation system. It recruits NEDD8 and its conjugates to the proteasome for degradation. It also interacts with PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome.
Background Reference 1:
Tanaka, T., et al., J. Biol. Chem. 278(35):32905-32913 (2003).
Background Reference 2:
Akey, D.T., et al., Hum. Mol. Genet. 11(22):2723-2733 (2002).
Background Reference 3:
Kito, K., et al., J. Biol. Chem. 276(23):20603-20609 (2001).
Background Reference 4:
Scanlan, M.J., et al., Int. J. Cancer 83(4):456-464 (1999).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-405

List Size:
400 ul

List Price:

$495.00

Datasheet
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